Effects of temperature on success of scale autografts and allografts in <i>Poecilia reticulata</i>

Abstract Acceptance or rejection of transplanted tissues is dependent upon both genetic and environmental factors. We used guppy fish, Poecilia reticulata, as a model for studying those factors impacting the immune response to scale tissues. Scale tissue acceptance observed when blood has begun circulate throughout grafted can occur at different time intervals due degree difference between donor recipient. Individual fish were genotyped MHC class I (UBA) II (DAA DAB) similarity underwent autografts genetically distinct allografts, followed by recovery period with temperatures. Fish exhibit behavioral fever under infection stress models, expected show levels inflammation graft success set tank temperatures 20, 23, 26 degrees Celsius (n = 5 per tank) monitored subjects five weeks following procedure observe signs chronic rejection. Typically, takes two three become evident, longer confirm grafts. rejection, including necrosis loss pigmentation, higher rates in allografts than autografts.